Immunodiagnosis of Haemonchus contortus infection in sheep by counter immuno-electrophoresis using somatic antigen
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چکیده
منابع مشابه
Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملimmunodiagnosis of haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (elisa)
indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of h. contortus. plate elisa was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. indirect plate elisa was able to demonstrate...
متن کاملImmunological responses of sheep to Haemonchus contortus.
Infections with Haemonchus contortus are a major constraint on ruminant health world-wide. Young lambs are very sensitive to Haemonchus infection. Older lambs and sheep acquire immunity after a continuous or seasonal exposure to the parasite. The mechanisms underlying immunity are still not completely understood. Antibodies, in particular local IgA and IgE, certainly play a role. The role of Ig...
متن کاملEarly detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
Haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (FOB). The objective was to study three different FOB tests in order to have a new indicator of H. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selection criterion to faecal worm egg count (FEC). A total of 29 Corriedale lambs were experimentally...
متن کاملImmunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
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ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2016
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-016-0801-6